Part 1 Basic techniques of biochemistry and molecular biology
Chapter 1 Basic techniques of preparation of biomacromolecules
Section 1 Salting out
Section 2 Dialysis and ultrafiltration
Chapter 2 Spectrophotometry
Section 1 Basic principles
Section 2 The basic construction of spectrophotometers
Section 3 Some home-made spectrophotometers
Chapter 3 Chromatography
Section 1 Adsorption chromatography
Section 2 Partition chromatography
Section 3 Ion-exchange chromatography
Section 4 Gel filtration
Section 5 Affinity chromatography
Chapter 4 Electrophoretic techniques
Section 1 Basic principles
Section 2 Several types of commonly used electrophoresis
Chapter 5 Centrifugation techniques
Section 1 Basic principle
Section 2 General description of centrifugation
Chapter 6 Genetic engineering and analytieal technology of gene expression
Section 1 Genetic engineering
Section 2 Polymerase chain reaction
Section 3 Nucleic acid hybridization
Section 4 DNA sequencing technology
Part 2 Experiments of the biochemistry and molecular biology
Chapter 7 Analysis of purification and physicochemical properties of proteins
Experiment 1 Colorimetric methods for protein determination
Experiment 2 Isolation and purification of serum y-globulin
Experiment 3 Cellulose acetate membrane electrophoresis of serum proteins
Experiment 4 Determination of relative molecular mass of protein in SDS-PAGE
Chapter 8 Kinetics of enzyme-catalysed reactions
Experiment 1 The specificity of enzyme and effect of temperature, pH on the enzyme Activity
Experiment 2 Isolation of alkaline phosphatase and determination of its specific activity
Experiment 3 Effects of substrates concentration of enzymatic activity: determination of Km of alkaline phosphatase
Experiment 4 Inhibition effect of phosphate on alkaline phosphatase
Experiment 5 Analysis of lactic dehydrogenase isoenzyme
Chapter 9 Substance metabolism
Experiment 1 Extraction and identification of liver glycogen
Experiment 2 Determination of alanine transaminase activity （Reitman-Frankels''method）
Experiment 3 Enzyme catalyzed transamination of amino acids detected by paper chromatography
Chapter 10 Basic experiment of molecular biology
Experiment 1 Preparation of the genomic DNA in the eukaryotic cells
ExPeriment 2 Determination of the concentration and purity of DNA by spectroscopy
Experiment 3 Agarose gel electrophoresis of nucleic acids
Experiment 4 Polymerase chain reaction （PCR）
Experiment 5 Isolation and purification of the DNA fragments
Experiment 6 DNA ligation--constructing recombinant plasmids
Experiment 7 Preparation of E.coli competent cells
Experiment 8 Transformation of Ca2+ competent ceils
Experiment 9 The screening of recombinant DNA
Experiment 10 Minipreparation of plasmid DNA
Experiment 11 Digestion of plasmid DNA with restriction endonucleases
Experiment 12 Southern blot
Experiment 13 Expression of exogenous gene in prokaryotic cells
References
Appendix
Appendix I The calculation table of ammonium sulfate saturation
Appendix II Common buffer solution preparation
Appendix III The relative molecular mass and pI of protein
Appendix IV Common data of chromatography
Appendix V The conversion between centrifugation speed of centrifugation force
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