Preface for Chinese Version of Book
It is a great pleasure for myself,and my co-editors of Bacteriophages:methods and protocols,that so many of the chapters that we commissioned and edited have now been translated into Chinese within this volume.Myself,and professors Andrew Kropinski and Rob Lavigne were aware when we started this project in 2005,that if we did not ‘save’ many of the protocols and methodologies that were being used by practitioners of phage biology,that they would be lost to history. This is because the leaders in the field were generally about to retire,and their protocols were dispersed in many discrete papers or not even written down formally. Furthermore,at the time although a few young research scientists coming into the field of bacteriophage biology,they did not have their own independent positions and so were not actively using or collating such information.
I am happy to say that almost two decades later the situation has completely reversed. The need and awareness to use bacteriophages has increased and expanded,which is reflected in funding and research initiatives within universities and companies across the world. There are also many new and thriving research groups dedicated fully on phage.The core techniques involved in working with bacteriophages were developed over decades and it is clearly extremely useful to build on these rather than having to reinvent them. This book hopes to support this by bringing you well ‘homed’ and fine-tuned methods to investigate all aspects of phage biology.
In addition to saving the traditional protocols,we also tried to ensure we captured many new methods as they were developed. We include in this book many cutting-edge and novel approaches that have allowed the field of bacteriophage biology to be really revolutionised. Our ability to sequence the genomes,transcriptomes and proteins of these organisms,and carry out work to understand the biology at a mechanistic level is unparalleled.Furthermore,our ability to explore issues surrounding the production and formulation of phages is now possible.
Although bacteriophages were discovered over a century ago and initially developed in the 1920s-30s,in light of the technical advancements in molecular biology and genetics,many of which we present here,it is likely that the 2020s-2030s will be the true ‘age of phage’.Therefore,phages will be able to be developed in a safe way which is fully conversant with an understanding of their biology.
We are happy that the work we did in gathering this material has been so meticulously translated by our Chinese colleagues,particularly Professor Yuqing Liu and his extended team,and we are grateful for all the work they did to do this. Global problems need global solutions,and we are pleased that our Chinese colleagues will have full access to this material. We wish you all the best of wishes and joy in discovering these protocols and in understanding,taming and exploiting your bacteriophages to prevent and control disease.